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Cloning, genomic organisation and mRNA expression of a pectin lyase gene from a mutant strain of Penicillium occitanis.

Identifieur interne : 000035 ( Main/Exploration ); précédent : 000034; suivant : 000036

Cloning, genomic organisation and mRNA expression of a pectin lyase gene from a mutant strain of Penicillium occitanis.

Auteurs : Hèla Trigui-Lahiani [Tunisie] ; Ali Gargouri

Source :

RBID : pubmed:17107764

Descripteurs français

English descriptors

Abstract

The regulatory cis elements of fungal pectinases are well studied in Aspergillus genera but little is known in other fungal species. A genomic bank from Penicillium occitanis fungus is constructed and screened by previously isolated cDNA probe of a pectin lyase. From several isolated clones, the nucleotide sequence of the pectin lyase gene was completed and led to the identification of introns and promoter-terminator regions. A streaking future was found in pnl gene of P. occitanis: it exhibits the highest nucleotide homology with the pnlA of Aspergillus niger but the positions of its 4 introns is completely identical to that of A. niger pnlB gene. In addition to the determination of transcription start site, the promoter sequence from the pnl gene was analysed. It showed the conservation of known consensus sequences -CreA, Hap2-3-4, PacC ...-, and the existence of a particular sequence -CCTGA- which is similar to that already found to be specific of pectinolytic gene in Aspergillus, CCCTGA. This result suggests that the corresponding regulatory trans-acting factor should be the same as in Aspergillus.

DOI: 10.1016/j.gene.2006.09.022
PubMed: 17107764


Affiliations:


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Le document en format XML

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<name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Hèla" last="Trigui-Lahiani">Hèla Trigui-Lahiani</name>
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<nlm:affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, BP K 3038-Sfax, Tunisia.</nlm:affiliation>
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<term>3' Flanking Region (MeSH)</term>
<term>5' Flanking Region (MeSH)</term>
<term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Blotting, Northern (MeSH)</term>
<term>Blotting, Southern (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Codon (MeSH)</term>
<term>DNA, Fungal (analysis)</term>
<term>DNA, Fungal (genetics)</term>
<term>Exons (MeSH)</term>
<term>Gene Expression Regulation, Enzymologic (MeSH)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Genomic Library (MeSH)</term>
<term>Introns (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Penicillium (enzymology)</term>
<term>Penicillium (genetics)</term>
<term>Phylogeny (MeSH)</term>
<term>Polysaccharide-Lyases (genetics)</term>
<term>Polysaccharide-Lyases (metabolism)</term>
<term>RNA, Fungal (genetics)</term>
<term>RNA, Fungal (metabolism)</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Transcription Initiation Site (MeSH)</term>
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<term>ADN fongique (analyse)</term>
<term>ADN fongique (génétique)</term>
<term>ARN fongique (génétique)</term>
<term>ARN fongique (métabolisme)</term>
<term>ARN messager (génétique)</term>
<term>ARN messager (métabolisme)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Banque génomique (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Codon (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Exons (MeSH)</term>
<term>Introns (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Penicillium (enzymologie)</term>
<term>Penicillium (génétique)</term>
<term>Phylogenèse (MeSH)</term>
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<term>Polysaccharide-lyases (métabolisme)</term>
<term>Région 3' flanquante (MeSH)</term>
<term>Région 5' flanquante (MeSH)</term>
<term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Site d'initiation de la transcription (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Technique de Northern (MeSH)</term>
<term>Technique de Southern (MeSH)</term>
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<term>DNA, Fungal</term>
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<term>RNA, Messenger</term>
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<term>RNA, Fungal</term>
<term>RNA, Messenger</term>
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<term>Penicillium</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en">
<term>Penicillium</term>
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<term>5' Flanking Region</term>
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<term>Cloning, Molecular</term>
<term>Exons</term>
<term>Gene Expression Regulation, Enzymologic</term>
<term>Gene Expression Regulation, Fungal</term>
<term>Genomic Library</term>
<term>Introns</term>
<term>Molecular Sequence Data</term>
<term>Mutation</term>
<term>Phylogeny</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
<term>Transcription Initiation Site</term>
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<term>Clonage moléculaire</term>
<term>Codon</term>
<term>Données de séquences moléculaires</term>
<term>Exons</term>
<term>Introns</term>
<term>Mutation</term>
<term>Phylogenèse</term>
<term>Région 3' flanquante</term>
<term>Région 5' flanquante</term>
<term>Régulation de l'expression des gènes codant pour des enzymes</term>
<term>Régulation de l'expression des gènes fongiques</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Site d'initiation de la transcription</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Technique de Northern</term>
<term>Technique de Southern</term>
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<front>
<div type="abstract" xml:lang="en">The regulatory cis elements of fungal pectinases are well studied in Aspergillus genera but little is known in other fungal species. A genomic bank from Penicillium occitanis fungus is constructed and screened by previously isolated cDNA probe of a pectin lyase. From several isolated clones, the nucleotide sequence of the pectin lyase gene was completed and led to the identification of introns and promoter-terminator regions. A streaking future was found in pnl gene of P. occitanis: it exhibits the highest nucleotide homology with the pnlA of Aspergillus niger but the positions of its 4 introns is completely identical to that of A. niger pnlB gene. In addition to the determination of transcription start site, the promoter sequence from the pnl gene was analysed. It showed the conservation of known consensus sequences -CreA, Hap2-3-4, PacC ...-, and the existence of a particular sequence -CCTGA- which is similar to that already found to be specific of pectinolytic gene in Aspergillus, CCCTGA. This result suggests that the corresponding regulatory trans-acting factor should be the same as in Aspergillus.</div>
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